📝 (I) Dilution method (1) Before testing, the sample should be homogenized using a homogenizer to a minced meat consistency. (2) Accurately weigh 1.00±0.05 g of homogenized fresh egg sample into a 10 mL centrifuge tube, add 4 mL of sample diluent, vortex for 2 min to mix thoroughly, obtaining the test solution. (II) Nitrogen blowing method 1. Break the fresh egg into a 100 mL beaker, mix thoroughly (egg white and yolk evenly mixed). 2. Weigh 2.00±0.05 g of homogenized fresh egg sample into a 10 mL centrifuge tube, add 0.5 mL NaCl solution, vortex for 2 min, then add 3 mL of sample extractant, vortex for 2 min, centrifuge at 3000~4000 r/min for 5 min. 3. Transfer 2 mL of the upper organic phase to a 10 mL centrifuge tube, blow dry in a 50~60°C water bath under nitrogen or air flow. Add 300 μL of sample diluent, vortex for 30 s to obtain the sample solution to be tested.

(I) Dilution method (1) Before testing, use a homogenizer to grind the sample into a meat paste. (2) Accurately weigh 1.00±0.05 g of homogenized fresh egg sample into a 10 mL centrifuge tube, add 4 mL of sample diluent, and vortex for 2 min to mix thoroughly, obtaining the test solution. (II) Nitrogen blowing method 1. Break the fresh eggs into a 100 mL beaker and mix thoroughly (stir egg white and yolk together). 2. Weigh 2.00±0.05 g of homogenized fresh egg sample into a 10 mL centrifuge tube, add 0.5 mL of NaCl solution, vortex for 2 min, then add 3 mL of sample extractant, vortex for 2 min, and centrifuge at 3000–4000 r/min for 5 min. 3. Transfer 2 mL of the upper organic phase into a 10 mL centrifuge tube and evaporate to dryness under a nitrogen or air flow in a 50–60°C water bath. Add 300 μL of sample diluent and vortex for 30 s to obtain the test sample solution.

🧪 Test Procedure

1. Please read the operating instructions carefully before starting the experiment. Allow the test strip and test sample to return to room temperature before use. 2. Remove the test strip from its original packaging and use it within 60 min after opening. 3. Using a pipette, add 100 μL of test sample solution (or use a 3 mL dropper to add 3 drops of test sample solution) into the sample well (S well). Start timing when the liquid begins to flow. Allow to react for 8 min, then interpret the result according to the schematic diagram; results read at other times are invalid. Read the result using a reader; results read at other times are invalid.