📝 Homogenize the tissue sample using a homogenizer. Weigh 2.00±0.05 g of the homogenized tissue sample into a 15 mL centrifuge tube, add 4 mL of extraction solvent, vortex for 2 min, and centrifuge at 4000 r/min for 5 min. Transfer 3 mL of the supernatant into a 10 mL centrifuge tube and evaporate to dryness under a nitrogen or air stream in a 50–60°C water bath. Add 200 µL of sample reconstitution solution, vortex for 20 s to obtain the test solution (for shrimp samples, add a 250 μL pipette tip during vortexing to aid drug dissolution, or pipette repeatedly 3–4 times).

Homogenize the tissue sample using a homogenizer. Weigh 2.00±0.05 g of the homogenized tissue sample into a 15 mL centrifuge tube, add 4 mL of extraction agent, vortex for 2 min, and centrifuge at 4000 r/min for 5 min. Transfer 3 mL of the supernatant into a 10 mL centrifuge tube and dry under a nitrogen or air stream in a 50–60°C water bath. Add 200 µL of sample reconstitution solution, vortex for 20 s to obtain the test solution (for shrimp samples, add a 250 μL pipette tip during vortexing to aid drug dissolution, or pipette repeatedly 3–4 times).