📝 Homogenize the tissue sample using a homogenizer. Weigh 2.50±0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 4 mL of Reagent A and 150 µL of derivatization reagent, and shake the sample up and down until it becomes a paste. Place the centrifuge tube in an 80°C water bath and incubate for 10 min. Remove the centrifuge tube, add 1 mL of Reagent B, cap the tube, mix, and cool to room temperature (20–25°C); add 5 mL of extraction solvent, cap the tube, and invert the tube 20–25 times. Do not vortex. Centrifuge at 4000 r/min for 5 min (if the supernatant after centrifugation is less than 3 mL, shake the sample up and down 3–4 times and centrifuge again). Transfer 3 mL of the upper layer to a 10 mL centrifuge tube and dry under a nitrogen or air stream in a 50–60°C water bath. Add 0.8 mL of cleanup agent, vortex for 1 min, then add 500 µL of sample reconstitution solution, and vortex for 5–6 s to mix thoroughly. Centrifuge at 4000 r/min for 5 min. It is strongly recommended to remove the upper organic phase. The remaining clear liquid is the test solution, which should be analyzed immediately.

Homogenize the tissue sample using a homogenizer. Weigh 2.50 ± 0.05 g of the homogenized sample into a 15 mL centrifuge tube. Add 4 mL of Reagent A and 150 µL of derivatization reagent. Shake the sample up and down until it forms a paste. Place the centrifuge tube in an 80°C water bath and incubate for 10 min. Remove the centrifuge tube, add 1 mL of Reagent B, close the lid, mix, and cool to room temperature (20–25°C). Add 5 mL of extraction agent, close the lid, and invert the tube 20–25 times. Do not vortex. Centrifuge at 4000 r/min for 5 min (if the supernatant after centrifugation is less than 3 mL, shake the sample up and down 3–4 times and re-centrifuge). Transfer 3 mL of the upper layer to a 10 mL centrifuge tube and dry under a nitrogen or air stream in a 50–60°C water bath. Add 0.8 mL of cleaning agent and vortex for 1 min. Then add 500 µL of sample reconstitution solution and vortex for 5–6 s to mix thoroughly. Centrifuge at 4000 r/min for 5 min. It is strongly recommended to remove the upper organic phase. The remaining clear liquid is the test solution and should be tested immediately.

🧪 Test Procedure

Before testing,, Please Firstread the operating instructions carefully. Before testing, read the operating instructions carefully. Before use, equilibrate the test card and the test solution to room temperature. Remove the test card from its original packaging, label it, and use it immediately. Using a micropipette, transfer 75 µL of the test solution and vertically dispense it into the sample well (S well), or use the disposable dropper provided in the kit to vertically and slowly add 2~3 drops of the test solution into the sample well (S well). Start timing when the liquid begins to flow. After a reaction time of 10 min, read the result immediately according to the schematic diagram or using a colloidal gold analyzer. Results read at other times are invalid.