📝 1. Homogenize the tissue sample using a homogenizer. 2. Weigh 4.00±0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 1 mL of extraction solvent 1, then add 5 mL of extraction solvent 2, vortex for 2 min, and centrifuge at 3000~4000 r/min for 5 min. 3. Transfer 3 mL of the upper layer into a 10 mL centrifuge tube and dry under a nitrogen or air stream in a water bath at 50~60°C. 4. Add 300 μL of sample reconstitution solution, vortex for 30 s, and the resulting solution is the sample to be tested.

1. Homogenize the tissue sample using a homogenizer. 2. Weigh 4.00±0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 1 mL of Extraction Reagent 1, then add 5 mL of Extraction Reagent 2, vortex for 2 min, and centrifuge at 3000~4000 r/min for 5 min. 3. Transfer 3 mL of the supernatant to a 10 mL centrifuge tube, and evaporate to dryness under a nitrogen or air stream in a 50~60°C water bath. 4. Add 300 μL of sample reconstitution solution, vortex for 30 s, and the resulting solution is the test sample solution.

🧪 Test Procedure

1. Before the experiment, please carefully read the operating instructions. Before use, allow the test card and the test sample to equilibrate to room temperature (20–25°C). 2. Remove the reagent bucket and test card bag from the original packaging. After opening, take out the required number of micro-well reagents and test cards, mark them, and use immediately. 3. Using a pipette, add 100 μL of the test sample liquid into the micro-well, slowly aspirate and mix thoroughly with the micro-well reagent. 4. After incubation at room temperature for 3 min, use a pipette to vertically add approximately 100 μL of the mixed solution into the sample well (S well), or use the disposable dropper provided in the kit to slowly and vertically add 3 drops of the mixed solution into the sample well (S well). 5. Begin timing when liquid starts to flow. Allow the reaction to proceed for 5 min. Immediately interpret the results according to the schematic diagram or read the results using a colloidal gold analyzer. Interpretation at other times is invalid.