Sample Prep

(1)homogenized tissue sample.

Sample Prep2

Before testing, the sample should be restored to room temperature. Weigh 2.00±0.05 g of sample into a 15 mL polystyrene centrifuge tube, then add 3 mL of extraction solution, close the lid, vortex or manually shake up and down for 1 min, and let stand for 1 min to obtain the test liquid. Note: After sample preparation is complete, spotting should be done within 5 min.

Sample Prep3

(3)取 3mLupper layerSolutionBody to 10 mL Centrifuge Tube , 于5060cwater bathnitrogen gas流orairFlow Downevaporate to dryness.

Sample Prep4

(4) Add 300 µL of purification agent, vortex with a vortex mixer for 60 s, then add 300 µL of sample reconstitution solution, and gently shake for 5 s.

Sample Prep5

(5) Centrifuge at 4000 r/min for 5 min. Take the upper organic phase; the lower aqueous phase is used as the sample solution.

Detection

before the experiment, Before the experiment, carefully read the instructions. Before use, allow the test card and sample to equilibrate to room temperature. Remove the reagent barrel and test strip bag from the original packaging. Open and take out the required number of microwell reagents and test strips, label them, and use immediately. Using a micropipette, aspirate 100 μL of sample solution into the microwell, slowly aspirate and mix thoroughly with the microwell reagent. After incubation at room temperature (20–25°C) for 3 min, aspirate approximately 100 μL of the mixed solution and vertically drop it into the sample well (S well). Start timing when liquid begins to flow. React for 8 min. Interpret the result according to the diagram or read the test result using a colloidal gold analyzer. Interpretations at other times are invalid.